Figure 6.

Characterization of At1g52760, a homolog of AhMGAT. A, MGAT activity was determined under standard assay conditions with an increasing amount of protein for 15 min with [¹⁴C]MAG and 20 µm oleoyl-CoA. FFA, Free fatty acid. B, The time-dependent acylation of MAG with 2 µg of protein. Lane 0, Enzyme was added after stopping the reaction; lane B, enzyme fraction was boiled for 5 min, and assay was performed. C, A typical phosphor image of MGAT assay with [¹⁴C]oleoyl-CoA and α,β-MAG as substrates. D, Preference of acyl-CoAs. The assay was performed with 50 μm [¹⁴C]MAG with 20 μm of different acyl-CoAs. The values are means ± sd of three independent determinations.