Figure 7.

Map-based cloning of CFO1. A, Location of CFO1 on chromosome 1. B, Fine-mapping of the CFO1 locus. C, Genomic structure of CFO1/OsMADS32. The site of the mutation in cfo1 and the T-DNA insertion site of AF36 are shown. The cfo1 mutation lies within an RsaI restriction site. D, The cfo1 mutation caused a premature translation stop. E, Verification of the cfo1 mutation site in the F2 progeny of cfo1 and M32OE plants using a CAPS marker. F, Verification of the M32OE site in the F2 progeny of cfo1 and M32OE plants using specific markers. G, Flower phenotypes of wild-type, cfo1, M32OE, and F2 progeny with genotype M32OE_cfo1cfo1. WT, Wild type. Bars = 1,000 μm in G. [See online article for color version of this figure.]