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LNO1 functions as a nucleoporin protein in Arabidopsis. LNO1 can functionally complement the defect in the yeast mutant nup159. The full-length LNO1, the N-terminal domain (NTD), and LNO1 without the N-terminal domain (LNO1ΔNTD) were cloned into the vector pAS1 and transformed into the nup159Δ1-456 and nup159VI→EE mutants. Yeast cultures were serially diluted (10×, 20×, 100×, 200×, 1,000×, and 2,000×) and spotted on YPD plates.
