Figure 6.
LNO1 interacts with Arabidopsis DEAD-box helicase/ATPase and LOS4 in yeast two-hybrid assays. The N-terminal domain of LNO1 (NTD), full-length LNO1, and LNO1 with deletion of NTD (LNO1ΔNTD) were fused with the GAL4 DNA activation domain (AD), and LOS4 was fused with the GAL4 DNA binding domain (BD). pACT2 (AD) and pAS1 (BD) empty vectors were included as negative controls. Yeast transformants were spotted on synthetic dropout plates without Leu (L) and Trp (W) or without Leu, Trp, and His (H) and with 20 mm 3-aminotriazole (3-AT). The 5-bromo-4-chloro-indolyl-β-d-galactopyranoside filter-lift assay was as described (Rea et al., 2012). Yeast cultures were serially diluted (10×, 100×, and 1,000×). [See online article for color version of this figure.]