Table I. The effect of ODD cofactors on the conversion of [14C]GA4 to [14C]GA7 by recombinant DES in vitro.
Final concentrations are as follows: 4 mm ascorbate, 4 mm 2-oxoglutarate, 5 mm FeSO4, 2 mg mL−1 bovine serum albumin, and 0.1 mg mL−1 catalase.
Cofactor Composition | Conversion |
---|---|
% | |
Full cofactor complement | 100 |
No cofactors | 0 |
2-Oxoglutarate | 19 |
Ascorbate | 0 |
FeSO4 | 0 |
Ascorbate, 2-oxoglutarate | 100 |
2-Oxoglutarate, FeSO4 | 82 |
Ascorbate, 2-oxoglutarate, FeSO4 | 100 |