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. 2012 Aug 21;160(2):837–845. doi: 10.1104/pp.112.201756

Table I. The effect of ODD cofactors on the conversion of [14C]GA4 to [14C]GA7 by recombinant DES in vitro.

Final concentrations are as follows: 4 mm ascorbate, 4 mm 2-oxoglutarate, 5 mm FeSO4, 2 mg mL−1 bovine serum albumin, and 0.1 mg mL−1 catalase.

Cofactor Composition Conversion
%
Full cofactor complement 100
No cofactors 0
2-Oxoglutarate 19
Ascorbate 0
FeSO4 0
Ascorbate, 2-oxoglutarate 100
2-Oxoglutarate, FeSO4 82
Ascorbate, 2-oxoglutarate, FeSO4 100