Table I. Levels of T6P, sugar phosphates, sugars, trehalose, and starch in TPP mutants.
Measurements were done in 12-d after germination plants grown on vertical plates supplemented with 1% Suc under continuous light. n = 4 independent experiments, ±sd. *, Data of shoot only; FW, fresh weight.
| Genotype/Metabolite | Col-0 | tppa | tppf | tppg | tppj | Col-0* | tppb* | tppi* | tpph* |
|---|---|---|---|---|---|---|---|---|---|
| T6P (nmol/g FW) | 0.104 ± 0.02 | 0.084 ± 0.01 | 0.079 ± 0.02 | 0.097 ± 0.01 | 0.104 ± 0.02 | 0.262 ± 0.02 | 0.180 ± 0.03 | 0.201 ± 0.03 | 0.272 ± 0.04 |
| Suc6P (nmol/g FW) | 0.365 ± 0.07 | 0.300 ± 0.04 | 0.323 ± 0.06 | 0.327 ± 0.03 | 0.329 ± 0.03 | 0.575 ± 0.09 | 0.516 ± 0.03 | 0.521 ± 0.06 | 0.443 ± 0.05 |
| Glc6P (nmol/g FW) | 199.4 ± 33.5 | 184.8 ± 9.2 | 177.4 ± 10.5 | 178.3 ± 26.5 | 187.1 ± 14.5 | 160.9 ± 18.7 | 192.1 ± 7.6 | 136.7 ± 11.7 | 167.7 ± 3.8 |
| Fru6P (nmol/g FW) | 57.6 ± 12.1 | 49.4 ± 3.8 | 46.8 ± 5.1 | 50.0 ± 6.6 | 52.7 ± 3.4 | 68.4 ± 1.3 | 73.7 ± 2.5 | 58.6 ± 3.3 | 57.7 ± 4.4 |
| UDPG (nmol/g FW) | 110.7 ± 16.7 | 109.8 ± 3.3 | 101.7 ± 1.6 | 102.8 ± 5.8 | 111.7 ± 10.2 | 54.1 ± 7.4 | 50.7 ± 2.4 | 43.3 ± 3.7 | 51.7 ± 7.1 |
| Suc (µmol/g FW) | 2.9 ± 0.4 | 2.7 ± 0.3 | 3.5 ± 0.7 | 3.3 ± 0.5 | 2.5 ± 0.4 | 1.9 ± 0.2 | 1.9 ± 0.2 | 4.5 ± 0.8 | 3.4 ± 0.7 |
| Glc (µmol/g FW) | 4.1 ± 0.3 | 4.5 ± 0.3 | 4.3 ± 0.2 | 4.7 ± 0.3 | 4.0 ± 0.2 | 5.2 ± 1.1 | 3.6 ± 0.1 | 6.6 ± 0.1 | 6.0 ± 0.2 |
| Fru (µmol/g FW) | 1.6 ± 0.1 | 1.9 ± 0.2 | 1.8 ± 0.2 | 1.8 ± 0.3 | 1.8 ± 0.3 | 2.7 ± 0.4 | 1.7 ± 0.5 | 4.2 ± 0.3 | 2.1 ± 0.8 |
| Trehalose (nmol/g FW) | 9.3 ± 1.9 | 7.9 ± 2.7 | 9.5 ± 2.4 | 7.7 ± 1.1 | 6.7 ± 1.3 | 22.9 ± 2.7 | 14.3 ± 3.4 | 21.2 ± 6.1 | 39.1 ± 8.2 |
| Starch (μmol gluc equivalents/g FW) | 14.6 ± 2.1 | 15.4 ± 1.9 | 11.4 ± 9.5 | 16.9 ± 7.7 | 15.5 ± 2.2 | 36.3 ± 3.9 | 29.7 ± 4.9 | 67.0 ± 11.6 | 42.7 ± 14.0 |