Arabidopsis NRT1.1 does not complement the M. truncatula nip-1 nodule phenotype. M. truncatula nip-1 and control wild-type composite plants transformed with pAtEF1α-AtNRT1.1, empty vector pCAMBIA2301 as a negative control, or pAtEF1α-MtNIP/LATD were grown as in Figure 5 with S. meliloti containing a constitutive lacZ gene. At 15 dpi, nodule characteristics were evaluated after staining with X-Gal for localization of rhizobia, which stain blue. A, A17 transformed with empty vector. B, A17 transformed with pAtEF1α-AtNRT1.1. C, A17 transformed with pAtEF1α-MtNIP/LATD. D, Mtnip-1 transformed with empty vector. E, Mtnip-1 transformed with pAtEF1α-AtNRT1.1. F, Mtnip-1 transformed with pAtEF1α-MtNIP/LATD. Bars = 200 μm.