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. 2012 Aug 17;160(2):944–954. doi: 10.1104/pp.112.200188

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© 2012 American Society of Plant Biologists. All Rights Reserved.

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Figure 1. — The physical association of SPRYSEC-19 and three SW5F variants in planta does not trigger a programmed cell death. A, Transient expression of SW5F variants (GCR161-1.1 and -1.2, and cv MoneyMaker) by agroinfiltration in N. benthamiana leaves together with empty expression vector (EV; left side of leaves) or SPRYSEC-19 (SS19; right side of leaves). Photographs were taken at 10 d post infiltration. B, Co-IP of HA-tagged LRR domains of the SW5F variants by 4MYC-SPRYSEC-19 or 4-MYC-GFP. SPRYSEC-19 and GFP were captured on anti-MYC agarose beads (IP MYC/IB MYC) in total protein extracts of agroinfiltrated leaves of N. benthamiana transiently coexpressing the proteins. LRR domains pulled-down by either 4-MYC-SPRYSEC-19 or MYC-GFP (IP MYC/IB HA) were detected on western blots with anti-HA serum. [See online article for color version of this figure.]