Figure 3.

SPRYSEC-19 suppresses SW5B activated programmed cell death. Leaves of N. benthamiana were agroinfiltrated with wild-type and autoactive mutant SW5 genes under 35S CaMV promoter and monitored for the initiation of cell death over 10 d. The suspensions of the bacteria carrying the constructs were infiltrated in a 1:1 ratio with a combined OD600 as indicated. Photographs were taken 5 d post infiltration. A, Wild-type (wt) SW5B and -F genes overexpressed next to SW5 mutants with a D-to-V mutation. B, Autoactive mutant SW5B(D857V) coinfiltrated with equal amounts of 4MYC-SPRYSEC-19 (left side of leaf) or 4MYC-GFP (right side of leaf).