Skip to main content
. 2012 Sep 23;2012:371379. doi: 10.1155/2012/371379

Table 1.

Oligonucleotide sequences.

Name Sequence (5′ to 3′)
Universal-OL1 GAAATTAATACGACTCACTATAGGGAAACCACTACGGTCTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG
GAGATATACCA
Myc-R AAGGTCTTCTTCACTAATCAGTTTCTGCTC
Universal-OL2 GAAATTAATACGACTCACTATAGGGAGACCGTAGTGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG
GAGATATACCA
FLAG-R CTTGTCGTCATCGTCCTTGTAGTC
Myc25A-R TTTTTTTTTTTTTTTTTTTTTTTTTAAGGTCTTCTTCACTAATCAGTTTCTGCTC
FLAG25A-R TTTTTTTTTTTTTTTTTTTTTTTTTCTTGTCGTCATCGTCCTTGTAGTC
PolyT oligo TTTTTTTTTTTTTTTTTTTTTTTTT
UnivOL1-F GAAATTAATACGACTCACTATAGGGAAACCACTACGGTC
UnivOL2-F GAAATTAATACGACTCACTATAGGGAGACCGTAGTGGTT
Fluo-F CAGGATCGAGTGGGTCAAAC
Fluo-R GGTGCTGGAGCTTTTGTCTG
p53-F GGCAGAGCTTGTAAGGTCAG
p53-R CAATCCATCCAATCCACTCC

The bold type resembles the sequences of the overlapping region for overlap-extension PCR.

Poly T oligo is labeled with a photo-cleavable biotin at the 5′ end.