Table 1.
Name | Sequence (5′ to 3′) |
---|---|
Universal-OL1 | GAAATTAATACGACTCACTATAGGGAAACCACTACGGTCTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG GAGATATACCA |
Myc-R | AAGGTCTTCTTCACTAATCAGTTTCTGCTC |
Universal-OL2 | GAAATTAATACGACTCACTATAGGGAGACCGTAGTGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG GAGATATACCA |
FLAG-R | CTTGTCGTCATCGTCCTTGTAGTC |
Myc25A-R | TTTTTTTTTTTTTTTTTTTTTTTTTAAGGTCTTCTTCACTAATCAGTTTCTGCTC |
FLAG25A-R | TTTTTTTTTTTTTTTTTTTTTTTTTCTTGTCGTCATCGTCCTTGTAGTC |
PolyT oligo | TTTTTTTTTTTTTTTTTTTTTTTTT |
UnivOL1-F | GAAATTAATACGACTCACTATAGGGAAACCACTACGGTC |
UnivOL2-F | GAAATTAATACGACTCACTATAGGGAGACCGTAGTGGTT |
Fluo-F | CAGGATCGAGTGGGTCAAAC |
Fluo-R | GGTGCTGGAGCTTTTGTCTG |
p53-F | GGCAGAGCTTGTAAGGTCAG |
p53-R | CAATCCATCCAATCCACTCC |
The bold type resembles the sequences of the overlapping region for overlap-extension PCR.
Poly T oligo is labeled with a photo-cleavable biotin at the 5′ end.