Figure 1. Rapamycin increases GSK3β phosphorylation at serine 9 in NSCLC cell lines. (A) the indicated cell lines were treated with 10 nmol/L rapamycin for 48 h. (B) A549-P and A549-RR cells were treated with rapamycin 1 nmol/L or 1 μmol/L as indicated for 24 h. After the aforementioned treatments, the cells were subjected to preparation of whole-cell protein lysates and subsequent western blot analysis for detection of the proteins as presented. D, DMSO; ra, rapamycin; ra1, rapamycin 1 nmol/L; ra2, rapamycin 1 μmol/L; L.E., longer exposure.