Table 2. Cell cycle modifications induced by ETP, CUR and their combination in MCF-7, HepG2, HCT116 and HeLa cells after 48 h of treatment.
| (A) MCF-7 cell line | ||||
|---|---|---|---|---|
| Groups |
Cell cycle phases |
|||
| SubG1 | G0/G1 | S | G2/M | |
| Control |
0.03 ± 0.001 |
89.40 ± 0.80 |
2.60 ± 0.01 |
8.00 ± 0.30 |
| ETP alone |
0.00 |
72.78 ± 0.60 |
18.19 ± 0.40 |
9.30 ± 0.20 |
| CUR alone |
0.01 ± 0.001 |
80.49 ± 0.40 |
13.78 ± 0.10 |
5.73 ± 0.30 |
| ETP + CUR | 0.00 | 63.17 ± 0.30a | 31.98 ± 0.50a | 4.85 ± 0.01 |
| (B)HepG2 cell line | ||||
|---|---|---|---|---|
| Groups |
Cell cycle phases |
|||
| SubG1 | G0/G1 | S | G2/M | |
| Control |
0.03 ± 0.001 |
70.86 ± 0.30 |
15.49 ± 0.04 |
13.65 ± 0.20 |
| ETP alone |
0.00 |
70.00 ± 0.05 |
22.23 ± 0.30 |
7.77 ± 0.60 |
| CUR alone |
0.05 ± 0.001 |
72.78 ± 0.07 |
12.84 ± 0.40 |
14.39 ± 0.06 |
| ETP + CUR | 0.00 | 68.62 ± 0.70 | 22.80 ± 0.20 | 8.58 ± 0.04 |
| (C) HCT116 cell line | ||||
|---|---|---|---|---|
| Groups |
Cell cycle phases |
|||
| SubG1 | G0/G1 | S | G2/M | |
| Control |
0.03 ± 0.001 |
87.50 ± 0.30 |
9.84 ± 0.70 |
2.66 ± 0.40 |
| ETP alone |
0.00 |
64.37 ± 0.30 |
34.00 ± 0.01 |
1.63 ± 0.02 |
| CUR alone |
0.00 |
72.97 ± 0.20 |
25.50 ± 0.08 |
1.53 ± 0.08 |
| ETP + CUR | 0.03 ± 0.001 | 64.81 ± 0.04 | 24.01 ± 0.10a | 11.17 ± 0.1a |
| (D) HeLa cell line | ||||
|---|---|---|---|---|
| Groups |
Cell cycle phases |
|||
| SubG1 | G0/G1 | S | G2/M | |
| Control |
0.00 |
72.24 ± 0.02 |
21.91 ± 0.07 |
5.85 ± 0.30 |
| ETP alone |
1.50 ± 0.01 |
63.11 ± 0.70 |
29.64 ± 0.05 |
5.75 ± 0.60 |
| CUR alone |
0.00 |
58.42 ± 0.08 |
41.35 ± 0.03 |
0.23 ± 0.02 |
| ETP + CUR | 8.03 ± 0.1a | 53.14 ± 0.10a | 20.57 ± 0.04a | 18.26 ± 0.04a |
Each point is the mean ± SEM. Values are represented as percentages of cells at the indicated cell cycle phases. aStatistical significance compared with ETP alone using one way ANOVA.