Figure 4. VE-821 perturbs the irradiation-induced cell cycle checkpoint in pancreatic cancer cells. VE-821 (1 µM) was added 1 h prior to 6 Gy irradiation and left for the duration of the experiment. Cells were lifted and fixed at 12 h or 24 h after irradiation, stained with propidium iodide and analyzed for cell cycle distribution by flow cytometry. Bar graphs represent mean +/− SEM (n = 6). Individual DNA histograms of representative samples are shown in Figure S2A.