Figure 3. Gadd45a decrease migration and invasion abilities of HepG2 cells in vitro. (A and B) The knockdown efficiency of Gadd45a siRNA was examined by Western-blot and Realtime-PCR. (C) Migration of cells through a layer of polycarbonate was determined according to the method above and incubated for 10 h. Invasion of cells are the same as the migration assay described above except that the filter was coated with 250 μl/ml Matrigel. (D-E)Ten fields were counted for each filter and results were representative of at least three separate experiments and were expressed as mean ± SD.