Figure 6. Analysis of Krt19 and Alcam promoter methylation by bisulfite sequencing. (A and B)Methylation status of Krt19 and Alcam gene in MEFs was analyzed, ten colonies of cloned BGS PCR products from each bisulfite-treated DNA sample were sequenced and each was shown as an individual row, representing a single allele of the promoter. Open circles denoted unmethylated CpG sites and filled circles represented methylated CpG sites, curved arrow represented transcription start site.