Table 3. Functional categories represented among deregulated proteins in IIB-BR-G-MTS6 vs IIB-BR-G.
| Annotation Tool | Functional Category | EASE* Score | Protein |
|---|---|---|---|
| GO Biological Process |
response to wounding |
9.07e-003 |
↑IL1α; IL1β; IL4; IL8, CCL17; CCL18; CCL20; CCL23; CXCL5; CCL3; CCL5; CCL8; CSF2; CSF3; CXCL1; ↓ CCL16; CXCL11; CXCL12 CCL19; CXCL9; CCL4; |
| GO Biological Process |
cell growth and/or maintenance |
7.82e-003 |
↑CCL23; CCL3; CCL5; PDGFA; LAP-TGFβ1 TIMP1; CCL8;CSF3;CXCL1;CXCL5; EGFR; FGF2; FGF9; HGF; IGFBP1; IGFBP3; IGFBP4; IGFBP6; IL1α; IL1β; IL4; IL6; IL6R; IL8; ↓OSM; BTC; CCL19 CCL4; CXCL12 FIGF; IFNγ |
| GO Biological Process |
chemotaxis |
2.37e-003 |
↑ CCL17; CCL18;CCL20; CCL23; CCL3; CCL5; CCL8; CXCL1; CXCL12; CXCL5; CXCL9; FGF2; IL1α; IL4; IL8 ↓ CCL16; CCL4;CCL19; CXCL11 |
| Organismal role |
Cell migration/motility |
2.36e-003 |
↑CCL17; CCL18; CCL20; CCL23; CCL3; CCL5; CCL8; CXCL1; CXCL12; CXCL5; CXCL9; FGF2; HGF; IFNγ; IGFBP3; IL4; IL8; PECAM1 ↓CCL16; CCL19; CCL4 CXCL11 |
| GO Biological Process |
response to stress |
4.11e-002 |
↑CCL17;CCL20; CCL23; CCL3; CCL5; CCL8; CSF2; CSF3; CXCL1; CXCL11; CXCL12; CXCL5; CXCL9; IL1α; IL1β; IL4; IL6; IL8 ↓CCL16; CCL18, CCL19, CCL4 |
| GO Molecular Function |
glycosaminoglycan binding/heparin binding |
2.92e-002 |
↑CCL23; CCL3; FGF9 |
| GO Molecular Function |
hydrolase activity/macromolecule catabolism |
1.77e-002 |
↑ANG; HGF; MMP1; MMP13; MMP9; TIMP1 |
| GO Biological Process |
regulation of cell proliferation |
1.56e-002 |
↑CCL23; CCL3; CSF3; CXCL1; CXCL5; FGF2; TGFβ1-LAP; TIMP1; IGFBP6; IL1α; IL1β; IL6; IL8; ↓ OSM; BTC;FIGF |
| GO Biological Process |
inflammatory response/ innate immune response |
1.47e-002 |
↑IL1α; IL1β; IL8; CCL17; CCL18; CCL20; CCL23; CCL3; CCL5; CCL8; CXCL1; CXCL11; CXCL5; ↓CXCL12; CCL4CXCL9; CCL16; CCL19; |
| GO Biological Process | cell-cell signaling | 1.29e-002 | ↑CCL17;CCL20; CCL23; CCL3; CCL5; CCL8; CSF3; CXCL5; FGF2; IL1α; LAP-TGFβ1;IL1β; IL6; IL8; PDGFA ↓CCL16; CCL4; FGF9; IFNγ; CXCL11; CXCL9; CCL18; CXCL12; TEK |
Functional categories were obtained using EASE software as described under Methods. Results correspond to total deregulated IIB-BR-G-MTS6 proteins (up- and downregulated) combining cell extracts plus CM results. EASE scores < 0.05 were considered significant. ↑ = protein upregulated in IIB-BR-G-MTS6 vs IIB-BR-G; ↓ = protein downregulated in IIB-BR-G-MTS6 vs IIB-BR-G.