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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: Cell Metab. 2012 Sep 5;16(3):336–347. doi: 10.1016/j.cmet.2012.07.017

Figure 1. Regulation of miRNA Expression in Adipose Tissue in Response to Age and Calorie Restriction (CR).

Figure 1

See also Figure S1 and Tables S1 and S6.

(A) Heat map representing the quantitation of 265 miRNAs expressed in the subcutaneous adipose tissue of mice at 1, 3, 6 and 24 months of age and at 6 months of age after 3 months of a 40% CR.

(B) and (C) Quantitation of selected miRNAs by SYBR-Green stem-loop PCR in subcutaneous fat of aged and calorie restricted mice. n=4–5 per group.

(D) RT-qPCR of the pri-, pre- and mature miR-145 and let-7e was performed as described in Experimental Procedures. n=5 per group.

* P < 0.05. Error bars, SEM. Ad Lib, Ad libitum. mo, months.