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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: Cell Metab. 2012 Sep 5;16(3):336–347. doi: 10.1016/j.cmet.2012.07.017

Figure 5. Evolutionary Conservation of miRNA Processing Pathway Regulation with Age in Human Preadipocytes and Worms.

Figure 5

See also Figure S5 and S6.

(A) and (B) Preadipocytes from humans [young (26±4.1 years), middle-aged (46±2.2 years) and old (74±3.7 years)] (n=3) were isolated. The mRNA levels of (A) the components of the miRNA processing pathway and (B) selected miRNAs were assessed by RT-qPCR. * P < 0.05 vs. young group. Values represent mean of 3 independent samples per age group, where each sample represents cells of a single individual. Variance among individuals for Dicer mRNA expression was 0.0032 (young), 0.0349 (middle-aged) and 0.0018 (old).

(C) RT-qPCR of Dicer mRNA in whole N2 (WT) and eat-2 (a genetic model of calorie restriction) worms at different ages.

(D) RT-qPCR of Dicer mRNA in whole N2 (WT) and hsf-1 overexpressing worms (hsf-1 o/e).

In worms, each point represent mean of at least 2 independent pools containing 30 worms each.

Error bars, SEM. * P < 0.05.