| Initial denaturation : | 1 min at 94°C (initial denaturation) |
| 30 cycles: | 1 min at 94°C (denaturation) |
| 1 min at 60°C (annealing) | |
| 1 min at 72°C (extension) | |
| Final extension: | 10 min at 72°C. |
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