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Two-step reaction scheme for synthesis of β6-20-PEGx-Vancomycin. Step 1 Combines MAL-PEGx-SCM with vancomycin in DMF/TEA, favoring formation of an amide bond at the vancomycin V3 amine. Step 2 combines MAL-PEGx-Vancomycin with Cys-β6-20 in PBS to create a stable thioether bond between the targeting peptide and the PEG linker.
