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. 2012 Sep 17;122(10):3579–3592. doi: 10.1172/JCI38576

Figure 7. P2ry12–/– mice were partially protected from OVX-induced bone loss.

Figure 7

OVX was performed on 14-week-old P2ry12–/– and WT littermates (n = 5 per group). (A) BV/TV and BMD were measured in the primary and secondary spongiosa of the tibia of live animals viva CT scanning prior to and at days 14 and 35 following OVX. (B) Serum concentration of CTX and P1NP measured by ELISA. (C) Bone formation was visualized by calcein (first) and alizarin red (second) double-labeling and visualized in the trabecular bone. Representative photographs: original magnification, ×2.5, scale bar: 300 μm; original magnification, ×20, scale bar: 50 μm. (D) MAR and BFR were measured. (E) TRAP-stained hind limb sections after OVX. Scale bar: 300 μm. (F) Quantification of OC and OB cells in the primary and secondary spongiosa of the femur. Data represent mean ± SD. n = 5. *P < 0.05.