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. 2012 Sep 4;122(10):3629–3634. doi: 10.1172/JCI60429

Figure 4. A SNP in the 3′ UTR of SLC26A9 reduces reporter gene activity in luciferase reporter assays.

Figure 4

(A) Alignment of the partial wild-type 3′ UTR sequence of SLC26A9 (nucleotides 4511–4529) with the SNP rs2282430 (indicated by arrowhead) and hsa-miR-632. (B) Summary of relative luciferase activity of reporter constructs containing the wild-type versus polymorphic (SNP) 3′ UTR of SLC26A9 transfected into A549 cells. (C) Effects of co-transfection with precursor hsa-miR-632 or a pre-miR negative control (miR-Ctrl) on luciferase activity of wild-type versus SNP SLC26A9 3′ UTR constructs in HEK293 cells. n = 10–11 experiments per group. *P < 0.05.