Figure 5. Deficiency in IL-1 signaling in IL-1R1–KOs ameliorates ALD.

WT or IL-1R1–KO mice were fed control (n = 5 per genotype) or alcohol (n = 10 per genotype) diet and sacrificed 4 weeks later. Liver injury was assessed by liver H&E staining and serum ALT (A). Steatosis was evaluated by Oil-red-O staining (B). Serum and liver levels of IL-1β (C), TNF-α (D), and MCP-1 (E) were measured using specific ELISA. Numbers in graphs denote P values. Original magnification, ×200.