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. 2012 Sep 10;122(10):3692–3704. doi: 10.1172/JCI61623

Figure 6. Severe depletion of functional hematopoietic stem cells in +9.5–/– embryos.

Figure 6

E12.5 fetal liver cells (CD45.2) from +9.5+/+, +9.5+/–, and +9.5–/– littermates were transplanted into irradiated CD45.1 recipient mice at a 1:1 ratio with CD45.2+ bone marrow cells. Peripheral blood was analyzed by flow cytometry 6 and 10 weeks after transplantation. (A) CD45.2+ cells in recipient mice expressed as percentage of total live nucleated cells. Data are presented as mean ± SEM. The contribution from +9.5+/– and +9.5–/– donors was significantly reduced compared with +9.5+/+ littermates as indicated (*P < 0.05). (B) The contribution of +9.5+/+ and +9.5+/– donor-derived CD45.2 cells to peripheral blood monocytes (Mac1+Gr-1–), granulocytes (Mac1+Gr-1+), T cells (Thy1.2+), and B cells (CD19+). At 10 weeks, significant increases in CD45.2+ T cells (P = 0.042) and B cells (P = 0.012) were observed in recipients of fetal liver cell transplants from +9.5+/– donors compared with +9.5+/+ littermate controls. Similar results were obtained in an additional transplantation experiment using 150,000 live nucleated fetal liver cells and 300,000 bone marrow cells (data not shown)