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. 2012 Sep 4;122(10):3603–3617. doi: 10.1172/JCI62229

Figure 8. TGF-β2 induces expression of TGF-β ligands and CAF markers in omental fibroblasts.

Figure 8

(A) Levels of TGF-β1 and TGF-β2 released by omental fibroblasts that were primed in SKOV3ip-conditioned medium or left unprimed, as in Figure 4C. Average results of ELISAs of 3 independent sets of each type of fibroblast-conditioned medium are shown. *P < 0.005; #P < 0.001. (B) Relative levels of TGFB1 and TGFB2 mRNAs (in human EOC cells) and Tgfb1 and Tgfb2 mRNAs (in mouse host cells) in omental tumors of mice that were inoculated with +HOXA9 control (Nontargeting) and HOXA9-knockdown (shA9-B) SKOV3ip lines (n = 5 mice per group). Specificity of human- and mouse-specific qRT-PCR primers is shown in Supplemental Figure 6B. *P = 0.025; †P = 0.018. (C and D) Relative TGFB1, TGFB2, ACTA2, and FAP mRNA levels in omental fibroblasts at 5 days after incubation with (C) recombinant TGF-β1 and TGF-β2 at the indicated concentrations and (D) media conditioned by +HOXA9 control SKOV3ip cells, where SKOV3ip-conditioned medium was left untreated, treated with IgG, or depleted by IP with Ab to TGF-β2.