Figure 5. Hsf1 deficiency attenuates MAPK signaling in NPcis mice.

(A) Immunoblotting demonstrated equivalent levels of total ERK, but less activation-associated p-ERK, in MPNSTs arising in Hsf1^–/– mice. Densitometric quantitation of immunoblots confirmed a statistically significant difference in mean ratio of p-ERK to total ERK (Student’s t test). (B) Basal p-ERK was diminished in NPcis^–/– MEFs derived from Hsf1^+/– and Hsf1^–/– embryos.