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. 2012 Sep 24;122(10):3516–3528. doi: 10.1172/JCI63352

Figure 8. At E17.5, pancreatic lineage cells remain unbranched and undifferentiated.

Figure 8

(A and B) The Rosa26:Tomato reporter (red) labels the Pdx1+ lineage in control (A) and DKO (B) pancreatic epithelium at E15.5. By this stage of development, the Pdx1+ progenies are restricted to a relatively unbranched single-cell–layered epithelium in the DKO pancreas. (C and D) Whole-mount and sections of LacZ-stained E17.5 control (C and E) and DKO (D and F) abdominal organs show well-extended glandular structure in the control pancreas (C) and only a small pancreatic remnant in the DKO (D). Sections through the remaining (ventral) pancreatic remnant in the DKO show LacZ-labeled Pdx1 progenies present in unbranched cystic ductal structures, and there is no morphological evidence of differentiated pancreatic tissue (F) as compared with the control sections (E). Note the extension of Pdx1-lineage–labeled cells in the proximal stomach in the DKO embryo (arrows in D). Original magnification, ×200 (A and B); ×20 (C and D); ×200 (E and F).