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. 2012 Sep 24;122(10):3791–3795. doi: 10.1172/JCI63937

Figure 2. Effect of PRL on gene expression and Kp immunostaining and GnRH release, and effect of Kp on PRL inhibition of GnRH release.

Figure 2

(A) Gene expression of pituitary Lhb and Fshb transcripts is decreased in PRL mice (n = 4) compared with control mice (n = 19) and increased in PRL+Kp mice (n = 8). **P < 0.01, *P < 0.05. (B) Gene expression of hypothalamic Gnrh transcripts is not altered among all 3 groups: control (n = 27), PRL (n = 10), and PRL+Kp mice (n = 10). (C) PRL inhibition of GnRH release from hypothalamic explants is reversed by 500 nM Kp. n = 10 for each group. **P < 0.01, *P < 0.05; see Methods for details. (D) Gene expression of Kiss1 transcripts is significantly decreased in PRL-treated (n = 10) compared with control (n = 26) mice. **P < 0.01. (E) Representative of 6 independent experiments. Kiss neurons staining in ARC and AVPV sections from control and PRL-treated mice. Scale bars: 50 μm. (F) Gene expression of hypothalamic Tac2 transcripts is not altered in the PRL-treated group of mice (n = 8) compared with the control group (n = 18).