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. 2012 Sep 10;122(10):3593–3602. doi: 10.1172/JCI64145

Figure 3. The ability of an HDAC inhibitor to enhance memory is blocked by the Nr4a dominant-negative transgene.

Figure 3

(A) Intrahippocampal injection of the HDAC inhibitor TSA (T) enhances acetylation of histone H3 (AcH3), as illustrated at 1 hour after TSA injection after training. Veh/V, vehicle. (B and C) Intrahippocampal TSA also increases expression of the (B) NR4A1 and (C) NR4A3 protein at the same time point. (D) Intrahippocampal TSA increases mRNA levels for each of the 3 Nr4a genes at 1 hour after injection. (E) Injection of TSA into hippocampi after training enhances 24-hour contextual fear memory of wild-type mice but fails to enhance memory of NR4ADN littermates. Error bars represent SEM. *P < 0.05. See also Supplemental Figure 3. Lanes were run on the same gel but were noncontiguous (white lines).