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. 2012 Sep 10;122(10):3541–3551. doi: 10.1172/JCI64151

Figure 3. Inhibition of miR-338-3p protects β cells against apoptosis.

Figure 3

Dissociated rat (A–C and E) and human (D) islet cells were transfected with anti-miRs (left) or oligonucleotide mimics (right) to modulate the expression of the indicated miRNAs. Cells were incubated for 24 hours with or without a mix of proinflammatory cytokines (10 ng/ml TNF-α, 0.1 ng/ml IL-1β, 30 ng/ml IFN-γ; A, B, D, and E) or for 48 hours either with 0.5% BSA or with 0.5% BSA coupled to 0.5 mmol/l palmitate (C). Apoptosis was assessed either by labeling the cells with annexin V (A) or by staining picnotic nuclei (B–E). Annexin V and propidium iodide staining allowed for assessment of apoptosis (gray bars) and necrosis (black bars), respectively. Data are mean ± SD of 4 independent experiments. *P < 0.05, ANOVA.