Skip to main content
. 2012 Sep 10;122(10):3541–3551. doi: 10.1172/JCI64151

Figure 8. Incretins signaling and miR-338-3p regulation.

Figure 8

Rat (A) or human (B) islets were incubated with or without 100 nM exendin-4 for 48 hours. miR-338-3p expression was then measured by qRT-PCR. (C) miR-338-3p expression was assessed by qRT-PCR in islets of wild-type and Glp1r–/–Gip1r–/– mice. Results in AC are expressed as percentage of U6. (D) Rat islet cells were transfected with a control oligonucleotide (white bars) or with an oligonucleotide leading to miR-338-3p overexpression (black bars). Igf1r and Irs2 expression were quantified by qRT-PCR. Results are mean ± SD of 4 independent experiments. *P < 0.05 vs. control, ANOVA. INS832/13 cells (E) and dispersed rat islet cells (F) were transfected with a control oligonucleotide or with an oligonucleotide leading to miR-338-3p overexpression. Cells were incubated in the absence or presence of 100 nM exendin-4 for 48 hours. Proliferation was assessed by scoring the fraction of BrdU+ (E) or Ki67+ (F) cells. Data are mean ± SD (n = 5 [E]; 6 [F]). *P < 0.05 vs. control, #P < 0.05 vs. exendin-4, ANOVA.