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. 2012 Sep 4;122(10):3490–3503. doi: 10.1172/JCI64906

Figure 3. Serotonin regulates FOXO1 transcriptional activity via its HTR1B receptor in osteoblasts.

Figure 3

(A) Representative vertebral section images from 2-month-old mice. Mineralized bone matrix is stained in black by Von Kossa reagent. Original magnification, ×4. (B) Representative 3D images of transverse CT slices of proximal tibia obtained by high-resolution μCT analysis. (C) In vivo osteoblast proliferation assessed in femoral sections expressed as the number of BrdU-stained osteoblasts per trabecular area or as the percentage of total osteoblasts per trabecular area (n = 3 mice/group). (D) Real-time PCR analysis of indicated genes in bones (n = 4 mice/group). (C and D) *P < 0.05 versus WT, #P < 0.05 versus Htr1b+/–Foxo1ob+/– group. (E) Calvaria cells from WT or Htr1b–/– mice transfected with FOXO-Luc and treated with vehicle or serotonin (100 μM) (n = 3). Mice were 2 months old. *P < 0.05 versus respective vehicle.