5HT significantly enhances CGRP release in female, but not male, human dental pulp. 5HT (100 μM; closed bars; n=35) compared to vehicle (open bars; n=32) does not have an effect on CGRP release alone, but does significantly enhance capsaicin 1 μM -evoked CGRP release from tooth pulp (males and females combined; A). When stratified by sex, there is no effect of 5HT on capsaicin-evoked CGRP release from male dental pulp (n=17) compared to vehicle (n=12; B). 5HT (n=18) significantly enhanced capsaicin-evoked CGRP release from dental pulp of females compared to vehicle (n=20; C). Capsaicin or vehicle alone induced a comparable release of CGRP from the dental pulp of males (open bars, n=12) and females (closed bars; n=20; D). * indicates significance at p<0.05 compared to vehicle.