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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2012 Jul 10;11(9):1936–1947. doi: 10.1158/1535-7163.MCT-12-0146

Figure 1.

Figure 1

KX-01 induced growth inhibition and apoptosis in ER/PR/HER2-negative breast cancer cells and synergized with paclitaxel in vitro. A) Chemical structures of KX-01, dasatinib and paclitaxel. B,C) Growth inhibitory effects of the broad specificity Src inhibitor dasatinib (B) and KX-01 (C) at varying concentrations (10, 25, 50, 100, 250nmol/L) on ER/PR/HER2-negative MDA-MB-231, MDA-MB-157, and MDA-MB-468 cells. Cells were incubated with drugs for 48h and growth inhibition was assessed by MTT assay. Results presented as % vehicle ± SD (n=3). D) KX-01 induced synergistic growth inhibition in MDA-MB-231 cells when combined with paclitaxel (PAX). Cell growth was measured by MTT assay. Cells were incubated with KX-01 (25nmol/L), PAX (5nmol/L), or combination for 24, 48, 72h. Results presented as % vehicle ± SD (n=3). E) KX-01 in combination with PAX resulted in enhanced apoptosis in MDA-MB-231 cells. Apoptosis assay was performed following 24h incubation with vehicle KX-01 (25nmol/L), PAX (5nmol/L) or KX-01+PAX. *, P<0.05 (Student's t-test), significantly different compared to vehicle, #, P<0.05 (Student's t-test), statistical evidence of enhanced apoptosis in combination treatment compared to either drug alone. Data are representative of three independent experiments.