Figure 2.

KX-01 inhibited growth of MDA-MB-231 xenografts. A) Athymic NUDE mice bearing MDA-MB-231 tumors (∼100mm³) were separated into three treatment groups randomly (n=10 tumors/group). The vehicle group received ultra-pure water and the other groups were treated with two different doses of KX-01 (1, 5mg/kg) for 28 days and tumor volumes were recorded as mm³ ± SD. *, P<0.05; (One-way ANOVA followed by Newman-keuls multiple comparison test). B,C) KX-01 inhibited phosphorylation of Src and downstream mediator FAK in MDA-MB-231 xenografts. IHC was performed as described in ‘Supplementary Methods’ for total Src, phospho-Src-Y416 (P-Y416-Src), FAK and phospho-FAK-Y861 (P-Y861-FAK). Representative photomicrographs are presented (200×). D-F) KX-01 induced apoptosis, and reduced proliferation and angiogenesis in MDA-MB-231 xenografts. Paraffin embedded tumor sections from vehicle and KX-01 (5mg/kg) groups were stained for TUNEL (D) and Ki67 (E). Fluorescent (TUNEL) and bright field (Ki67) photomicrographs were taken and representative images are presented (200×). F) Frozen tumor sections were stained for mouse CD31. Bright field microscope images (40×) were taken and representative photomicrographs are presented.