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. Author manuscript; available in PMC: 2013 Feb 23.
Published in final edited form as: Neuron. 2012 Aug 23;75(4):585–592. doi: 10.1016/j.neuron.2012.06.034

Figure 3.

Figure 3

ASK antagonism of ADL decreases C9 avoidance in males.

A. Wild-type males do not avoid C9. Avoidance assays were performed in the presence of food. n=20–70 animals each.

B. (Left) Changes in intracellular fluorescence in GCaMP3-expressing ADL neurons of wild-type hermaphrodites and males upon addition of 100 nM C9. n≥12 neurons each. Shading around the lines represents SEM. (Right) Average peak percentage change in fluorescence upon C9 addition. ** indicates responses different from wild-type at P<0.01. Also see Figures S3A and S3B.

C. ASK and RMG antagonize ADL in wild-type males. Avoidance of C9 in the presence of food. * and ** indicate responses different from wild type at P<0.05 and P<0.01, respectively. n=20–120 animals each. Also see Figure S3C.

D. C9 responses in wild-type male ADL neurons are unaffected by ASK ablation. Ca2+ transients upon C9 addition (red horizontal bar) in ADL in wild-type and ASK-ablated animals. n≥10 neurons each. Error bars in all panels represent SEM.