Skip to main content
. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Biochemistry. 2012 Aug 16;51(34):6847–6859. doi: 10.1021/bi300466d

Figure 4.

Figure 4

Fluorescence lifetime decay curves (ex=375, em=460) of 6-MI containing ssDNA and dsDNA molecules compared to monomer (black). Dynamic quenching of 6-MI occurs upon incorporation into ssDNA oligomers, as revealed by significantly faster decays (brown, dark green, pink, teal). The dsDNA molecules containing either the ATFAA or AAFTA sequences (TFA15ds-green, TFA07ds-blue, and cAFT10ds-magenta) exhibit fluorescence decays similar to 6-MI monomer (black), indicative of a lack of quenching. Notably, the AFT07 duplex exhibits a fast decay (red). The instrument response function is shown in gray.