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Equilibrium binding curves measured by fluorescence intensity with decreasing concentrations of 6-MI-containing DNA (exc = 340; em = 440 nm). DNA concentrations as low as 50 pM can accurately measure protein binding affinity using fluorescence intensity. Even at the lowest DNA concentration, binding is well determined as shown by the consistent dissociation constants obtained, (Kd = 2 ± 0.5nM) (Table 4).
