. Author manuscript; available in PMC: 2013 Aug 28.

Published in final edited form as: Biochemistry. 2012 Aug 16;51(34):6847–6859. doi: 10.1021/bi300466d

Table 3.

Parameters¹ derived from fitting of time-resolved anisotropy decay curves

DNA molecule	β₁² (±0.02)	θ_L (ns) (±0.06)	β₂ (±0.02)	θ_R (ns) (±0.9)
6-MI	-	-	-	-

AFT07ss	0.25	0.3	0.75	3.68
AFT07ds	0.2	0.61	0.8	8.8

TFA15ss	0.22	0.32	0.78	4.42
TFA15ds	0.09	1.66	0.91	16.81
TFA15ds + IHF	0.04	1.68	0.96	26.25

TFA07ss	0.33	0.13	0.67	3.27
TFA07ds	0.16	1.16	0.84	15.6
TFA07ds + IHF	0.1	1.18	0.91	25.99

cAFT10ss	0.22	0.42	0.78	5.24
cAFT10ds	0.13	1.41	0.87	17
cAFT10ds + IHF	0.1	0.9	0.9	24.78

Msh_TFA15ds	0.14	0.73	0.86	14.64
Msh_TFA15ds +Msh2-Msh6	0.16	2.11	0.84	20.73

Msh_TFA09ds	0.16	2.33	0.84	18.08
Msh_TFA09 +Msh2-Msh6	0.2	2.81	0.8	25.51

Fitting of anisotropy decay curves was done with the following expression: $r (t) = r_{0} (β_{1} e^{- (\frac{t}{θ_{L}})} + β_{2}) e^{- (\frac{t}{θ_{R}})}$

For all parameters errors are given in parentheses.