Figure 5.

Changes in the amount of transport of TfR and NgCAM during development in culture. In the case of carrier vesicles labeled with TfR-GFP, the number of anterograde transport events in stage 2 cells (square), which lack an axon, is roughly comparable to the number of events seen in dendrites throughout development (open circles). In contrast, the number of TfR-GFP-containing vesicles entering the developing axon drops abruptly when the cells enter developmental stage 3 (filled circles). In the case of NgCAM, the number of anterograde transport events in dendrites remains constant during development, whereas the number of NgCAM-GFP-containing vesicles entering the axon increases gradually during development. Anterograde transport events were quantified by using kymograph analysis and normalized for the duration of the recording and the length of the neurite included in the image. The data for mature cells (>14 days in culture) were taken from ref. 1.