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. 2012 Aug 10;12:141. doi: 10.1186/1471-2229-12-141

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Copyright ©2012 Ma et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The protein and mRNA levels of1Bx14in the grains of BSMV:1Bx14 infected spikes. Grain samples were collected at 20dpi from each wheat cv. infected with BSMV:1Bx14 or BSMV:00, and each of the grains was divided into two parts, one used for Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and the other for quantitative real-time PCR (qRT-PCR) analysis. (A) and (B) SDS-PAGE of HMW-GSs in the grain from cultivars Xiaoyan 6 or Shaanyou 225 inoculated with BSMV:00 or BSMV:1Bx14, respectively. Arrows indicate the reduced HMW-GS 1Bx14. (C) and (D) The scanning profiles of SDS-PAGE maps shown in Figure 6 and 6B. (E) and (F) QRT-PCR analysis of the relative expression of 1Bx14 in the same grain as in Figure 6A or 6B.