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. 2012 Aug 9;9:155. doi: 10.1186/1743-422X-9-155

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Copyright ©2012 Ch'ng et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cytopathic effects in Vero cells following a neutralization test. Mixtures of sera dilutions and EV71 strain A104 virus were assayed on Vero cells. Cytopathic effects were examined after 7 days of incubation and neutralization titers were determined. Pre-challenge sera showed the NPfl-immunized samples (1:8 dilution) and NPt-VP1_1-100-immunized samples (1:8 dilution). 1:512 dilution was used for the post-challenge and the positive control sera. No serum was used in the negative control samples. Magnification = 200X.