Table 1.
PCR primers
| Murine Gene | Primer sequence | Annealing temperature | Cycles |
|---|---|---|---|
|
Pgr |
5’ATGGTCCTTGGAGGTCGTAA3’ 5’CACCATCAGGCTCATCC3’ |
60 |
34 |
|
Esr1 |
5’CCAAAGCCTCGGGAATG3’ 5CTTTCTCGTTACTGCTGG3’ |
56 |
33 |
|
Esr2 |
5’GGGCCTGTTCGCCAGACTGC3’ 5’CAGGGATTTTCTTGGC3’ |
56 |
33 |
|
Ihh |
5’CGTGCATTGCTCTGTCAAGT3’ 5’CTCGATGACCTGGAAAGCTC3’ |
56 |
37 |
|
Pct1 |
5’CCTCCTTTACGGTGGACAAA 3’ 5’ GCCACATCAAGAGGTTTGGT 3’ |
56 |
40 |
|
Gli1 |
5’CCTGGTGGCTTTCATCAACT3’ 5’GTGGTACACAGGGCTGGAGT3’ |
56 |
38 |
| β-actin | 5’GATGACGATATCGCTGCGCTG3’ 5’GTACGACCAGAGGCATACAGG3’ | 60 | 26 |