Table 1.

Primer information

Primer	Sequence 5’-3’	Gene	Size (bp)
FluA F	AGGTGACACTATAGAATATTCTAACCGAGGTCGAAACG	M	270
FluA R	GTACGACTCACTATAGGGAACAAAGCGTCTACGCTGCAG
FLuB F	AGGTGACACTATAGAATAAAAAGRAGATTCATCACAGAGC	M	166
FLuB R	GTACGACTCACTATAGGGATTCTGCTATTTCAAATGCTTCA
sH1N1 F	AGGTGACACTATAGAATAGGTATGCTTTTGCAMTGARTAGAGG	HA	250
sH1N1 R	GTACGACTCACTATAGGGAAAGGGATATTCCTTARTCCTGTARCCAT
PIV1 F	AGGTGACACTATAGAATATCTCATTATTACCYGGACCAA	HA	284
PIV1 R	GTACGACTCACTATAGGGATCCTGTTGTCGTTGATGTCATA
PIV2 F	AGGTGACACTATAGAATATCTACACTGCATCAGCCAGC	HA	194
PIV2 R	GTACGACTCACTATAGGGACCCCTAAAAGAGATGAGCCC
PIV3 F	AGGTGACACTATAGAATATTGTCAATTATGATGGYTCAATCT	HA	230
PIV3 R	GTACGACTCACTATAGGGAGACACCCAGTTGTGTTGCAG
HRV F	AGGTGACACTATAGAATACCCCTGAATGYGGCTAACCT	5' UTR	144
HRV R	GTACGACTCACTATAGGGACGGACACCCAAAGTAGTYGGT
HMPV F1	AGGTGACACTATAGAATACATGCCCACTATAAAAGGTCAG	L	208
HMPV R1	GTACGACTCACTATAGGGACACCCCAGTCTTTCTTGAAA
HMPV F2	AGGTGACACTATAGAATAGAGCTAAYAGAGTGCTAAGTGATG	N	208
HMPV R2	GTACGACTCACTATAGGGAACTTTCTGCTTTGCTTCCTGT
Adv F	AGGTGACACTATAGAATAGCCSCARTGGKCWTACATGCACATC	Hexon	338
Adv R	GTACGACTCACTATAGGGACAGCACSCCICGRATGTCAAA
NL63 F	AGGTGACACTATAGAATATCCCAAATGTGATAGAGCTTTGC	Polym-erase	176
NL63 R	GTACGACTCACTATAGGGACTGTTAAAACTTGTGCCAACTC
OC43 F	AGGTGACACTATAGAATAATTGCACCAGGAGTCCCA	N	200
OC43 R	GTACGACTCACTATAGGGATATCGGTGCCGTACTGGTCT
229E F	AGGTGACACTATAGAATACTCGGAATCCTTCAAGTGACAGA	N	183
229E R	GTACGACTCACTATAGGGAACGAGAAGGCTTAGGAGTAC
HKU1 F	AGGTGACACTATAGAATATATAGTRAAACCTGATATGGCT	N	220
HKU1 R	GTACGACTCACTATAGGGATACCAAAACACTGTTGAACAT
RSVA F	AGGTGACACTATAGAATACATCCCCTCTATGCACAACC	F	158
RSVA R	GTACGACTCACTATAGGGACATGTTTCAGCTTGTGGGAA
RSVB F	AGGTGACACTATAGAATAAAACGAAGATTTCTGGGCTTC	F	279
RSVB R	GTACGACTCACTATAGGGATGCGACAGCTCTGTTGATTT
HBoV F	AGGTGACACTATAGAATAAAGAAAAGGGAGTCCAGAA	NP1	290
HBoV R	GTACGACTCACTATAGGGACTCTGTGTTGACTGAATACAG
Rnasep F	AGGTGACACTATAGAATAGAGGCCTGGCTTTTGAACTT	RNase P	125
Rnasep R	GTACGACTCACTATAGGGAATCAAATTGAGGGCACTGGA
Tag F	AGGTGACACTATAGAATA
Tag R	GTACGACTCACTATAGGGA

^a The primers HMPV F1 and HMPV R1 were reported previously [14]; the primers Adv F and Adv R were reported previously [15]; the primer HBoV R was reported previously [16]. The Universal Tag sequences are underlined.