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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Anal Biochem. 2010 Nov 5;409(2):195–201. doi: 10.1016/j.ab.2010.10.020

Figure 2. Secondary structure analysis and thermal stability of 5HB by Circular Dichroism spectroscopy.

Figure 2

Figure 2

(A) CD spectrum of 5HB in 10 mM PBS at pH 7.4. (B) The melting curve of 5HB obtained from the ellipticity measurements at 222 nm between 0 °C and 85 °C. ([Θ]: per residue molar ellipticity measured in degrees · cm2 · dmol−1 · residue−1)