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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Anal Biochem. 2010 Nov 5;409(2):195–201. doi: 10.1016/j.ab.2010.10.020

Figure 4. Binding titration curve of Fl-C35 and its binding stability to the 5HB.

Figure 4

Figure 4

(A) The fluorescence polarization response of Fl-C35 binding to the 5HB was monitored as the concentration of the 5HB increased. The experiment was performed using 5 nM Fl-C35 and the 5HB concentration ranged from 0 to 500 nM. (B) The stability of FL-C35 binding to the 5HB was monitored over a 24 hr period using 5 nM of Fl-C35 in the presence of increasing amount of the 5HB.