Table 1. Primer pairs used in quantitative reverse-transcriptase PCR on mouse tissue.
| Protein name | Acc. No | Forward primer (pmol/µL) | Reverse primer (pmol/µL) | T1 (°C) |
| Methionine synthase (MS) | NM_001081128 | 5’-GCAGATGTGGCCAGAAAAG-3’ (5) [23] | 5’-GCCACAAACCTCTTGACTC-3’ (5) [23] | 60 |
| Methylenetetrahydrofolate reductase (MTHFR) | NM_010840 | 5’-AGCTTGAATCCACCTGGACTGTAT-3’ (5) [23] | 5’-AGACTAGCGTTGCTGGTTTCAGA-3’ (5) [23] | 56 |
| Methylmalonyl-Coenzyme A mutase (MUT) | NM_008650.3 | 5’-GCAGGCTTTAGTACTGTGG -3’ (10) | 5’-CCAAGTCAAAGGCAACAGAC-3’ (10) | 60 |
| Lysosomal cobalamin transporter (LMBRD1) | NM_026719 | 5’-CTGGAGAACACGGAGGACAT-3’ (10) | 5’-GCTTTAAGGCACGTCTATCC-3’ (10) | 56 |
| Transcolabamin (TC) | NM_015749 | 5’-GATGTCCTGAAGTTGGCACA-3’ (5) [2](5) | 5’-TCCTGGGGTTTGTAGTCAGC-3’ (5) [2] (5) | 60 |
| TC-receptor (TC-R) | NM_19421.3 | 5’-GACTGCTCTGATGGCAGTGA-3’ (5) [2] (5) | 5’-GCCACGTGTGTGGAATACAG-3’ (5)[2] (5) | 60 |
| β-Actin | NM_007393.3 | 5’- AGGTGACAGCATTGCTTCTG -3’ | 5’-GCTGCCTCAACACCTCAAC-3’ | 60 |
1: Annealing temperatures used in q-rt-PCR for the given primer pair.