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. 2012 Oct 1;7(10):e46281. doi: 10.1371/journal.pone.0046281

Figure 4. VAPB is required for tumor growth in vivo.

Figure 4

(A) One million of MMTV-Neu control or VAPB knockdown cells were injected into cleared mammary gland fat pads of 3-week old FVB recipient female mice (n=8−10/experimental condition). Tumors were harvested 5 weeks after transplantation. Tumor size was measured by a caliper and tumor volumes were calculated. (p<0.05, ANOVA). (B) Tumors were processed, sectioned and stained with H&E (B) or Ki67, a proliferation marker (C). Proliferating tumor cells were quantified by enumeration of Ki67+ nuclei and presented as a percentage of Ki67+ nuclei/total nuclei. VAPB knockdown cells showed a significant decrease in proliferation (p<0.05, ANOVA). Arrows: Ki67 positive cells.