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. 2012 Oct 1;7(10):e46460. doi: 10.1371/journal.pone.0046460

Figure 4. Biological activity of human and mouse IL-10 variants on human and mouse macrophages.

Figure 4

Plant produced (p) and recombinant (r) E. coli produced human (h) or mouse (m) IL-10 were calibrated to contain the same amount of IL-10 as well as total soluble protein by using the empty vector control. Human (THP-1) and mouse (RAW264.7) macrophages were then pretreated with 10 ng/ml hIL-10 or mIL-10 for 20 min and subsequently stimulated with 1 µg/ml E. coli lipopolysaccharide. Tumor Necrosis Factor-alpha (TNF-α) expression was determined by ELISA and IL-10 activity is indicated as the percentage of inhibition of TNF-α expression as compared to the empty vector control (n = 3, error bars indicate standard error).