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. Author manuscript; available in PMC: 2012 Oct 2.
Published in final edited form as: Science. 2011 Aug 4;333(6047):1303–1307. doi: 10.1126/science.1210944

Fig. 1. Purified Tet2 catalyzes the modification of 5mC and 5hmC.

Fig. 1

(A) The 32P spot X on a TLC plate generated from 5mC and 5hmC DNA substrates incubated with the full-length Flag-Tet2 protein. The top spot in lanes 3 to 8 was 5′ end-labeled deoxyadenosine monophosphate (dAMP) resulting from the incomplete EcoNI digestion of the DNA substrate (fig. S1A). (B) TLC confirmation of the origin of spot X from 5mC with a 14C-labeled methyl group. (C) HPLC detection of a new nucleoside generated from 5mC and 5hmC DNA substrates upon incubation with Flag-Tet2. AU indicates absorption units.